The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users. 

• Cloning efficiency greater than 90% 

• IPTG and X-Gal are not required 

• Accepts a wide range of insert/vector ratios 0.5:1 to 12:1 

• Accepts insert size from 6 bp to 11 kb

• The phosphorylation of PCR fragments is not required

• Accepts blunt end amplicon or DNA fragment (not for sticky ends)

• Ampicillin and kanamycin selection markers

Store at  -20°C for 24 months