Trypto Soy Agar, otherwise known as Tryptic Soy Agar or Trypticase Soy Agar, is a general growth medium prepared with or without enrichments. It is used for the isolation of different strains of fastidious microorganism. TSA with 5% sheep blood is useful for the isolation and determination of hemolytic reactions of Staphylococcus spp., Streptococcus spp.