TUNAIR's Baffled Erlenmeyer Shake Flasks with 2-piece Caps, made from autoclavable polypropylene, are available with either 0.22µm Dry-Gauze nitrocellulose or 0.22µm silicone filter membrane cap liners for gas exchange without contamination. The flask's unique and patented baffle design and it's turbo-vane 2-piece cap closure system allows for superior gas exchange aeration for culture growth and productivity - Better than standard Erlenmeyer flasks. These baffled flasks are designed to enhance vertical and circular culture movement to improve aeration. The TUNAIR™ Flask's unique design increases the availability of dissolved oxygen, improving cell yields. Designed for microbiology and biotechnology applications, they provide for optimum growth conditions for aerobic microorganisms (ideal for E.Coli growth), mammalian cells, and plant cells.
TUNAIR™ baffled flasks, with increased growth surface area and improved areration, show superior growth when compared to standard flasks using microorganisms, including Escherichia coli, Saccharomyces cerevisiae, Penicillium avellaneum, and Streptomyces chartreusis. The aeration capacities of these baffled shake flasks, determined by the sulfate oxidation method, demonstrate superior areation and growth rates for these bacteria and others.
0.22 micron filters: Tunair Flasks are available with two different types of 0.22 micron filters (Dry-Gauze and Silicone). These filters are autoclavable for sterility and allow for maximum gas exchange without contamination. Dri-Gauze – This is a nitrocellulose mebrane filter than can be used approximately 6 to 8 times before having to be replaced. This filter can be autoclaved while in the cap, on the flask, for 6 to 8 times. Silicone – This is a silicone filter that will last the life of the flask. This filter can be autoclaved while in the cap and on the flask for many times.
Cap & Filter assembly: Once you have the 2-piece cap assembly apart (either the 300ml cap or the 2.5L cap) place the corresponding filter size inside the cap and re-assemble the inner and outer cap pieces, making certain to align the tabs with their associated holes. Once clipped into place, make certain that the filter completely covers the cap assembly openings.
Preparing and Cleaning the TUNAIR flask: Your TUNAIR flasks are constructed of polypropylene plastic which allows them to be autoclaved for sterility. Once you have prepared your media inside the flask, place the 2-piece cap with filter on the flask and insert into the autoclave. Set the autoclave for wet cycle. Once you have harvested your cells, wash the TUNAIR flask in a mild dish soap such as Dove Dish Soap then rinse well with DI water. Do NOT rinse the Dri-Gauze filter between uses. The silicone filter can also be rinsed with DI water if needed. Observe the condition of the filters between runs to make certain there are no holes or tears in the filter. If there are, replace the filter immediately.
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The Full-Baffle TUNAIR™ has six baffles that produce a propeller motion.
The working volume for the 300ml Full Baffle unit is 100ml, while the working volume for the 2.5 liter Full Baffle unit is 1 Liter.
The Half-Baffle TUNAIR™ has two baffles that produce a vortex motion.
The working volume for the 300ml Full Baffle unit is 100ml, while the working volume for the 2.5 liter Full Baffle unit is 1 Liter.
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- Dri-Gauze filters (Nitrocellulose) and Silicone filters are 0.22 micron pore. Dri-Gauze filter can be used 8-10 times before needing replacement.
- The Dri-Gauze filters can be autoclaved in the cap and left in place until a new filter is needed. The Dri-Gauze filters cannot be cleaned.
- The Silicone filters can be autoclaved while in the cap and on the flask for many times.
- The flasks are constructed of autoclavable polypropylene making them resistant to most solvents.
- TUNAIR™ flasks and caps can be cleaned by soaking in water with a light detergent solution to loosen particulates and contaminants, then air dry.