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The E.Z.N.A.® Endo-Free Plasmid Midi/Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA. Following lysis, endotoxins are removed from the cleared lysate with the addition of ETR Solution. Plasmid DNA is then further purified by adsorbing to the HiBind silica membrane. An easy wash step is followed by the elution of the bound plasmid DNA in elution buffer. Up to 200 µg of high-quality plasmid DNA can be purified from 30-50 mL of overnight culture (D6915), and up to 1 mg from 100-200 mL of overnight culture (D6926). Purified DNA samples are ready to be used in downstream applications such as, automated fluorescent DNA sequencing, restriction enzyme digestion, transfection of mammalian cells, cloning, and PCR amplification.

  • Speed – Purification of plasmid DNA in < 45min
  • Safety – No Phenol/chloroform extractions
  • Quality - Endotoxins efficiently reduced (<0.1 EU/µg)

D6915

Omega Biotek Endotoxin Free FastFilter Plasmid DNA Midi Kit, 2 preps

Endo-Free FastFilter Plasmid DNA Midi Kit, 2 preps (Description below)
SKU: D6915-00
Old price: $47.30
$46.15
Products specifications
Attribute nameAttribute value
Sample Size500-2000mL
Binding Capacity200 μg
Processing Time50 min
Plasmid TypeHigh & Low Copy Plasmids
Starting Amounts15-50 mL
Restriction Enzyme, Sequencing, PCR, TransformationYes
Transfection, In vitro transcriptionYes
Sensitive Cell Line & Primary Cell TransfectionYes
Lysate Clearance SyringeYes
Transfection and PCRYes